14 Mhando dzeHuman Papillomavirus ine njodzi huru (16/18/52 Typing)
Product name
HWTS-CC019A-Mhando gumi neina dzeHuman Papillomavirus ine njodzi huru (16/18/52 Typing) Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Ongororo dzakaratidza kuti HPV inoramba ichibata utachiona uye zvirwere zvakawanda ndicho chimwe chezvikonzero zvikuru zvegomarara remuchibereko.Parizvino, mishonga inozivikanwa inobudirira ichiri kushaikwa kwegomarara remuromo wechibereko rinokonzerwa neHPV, saka kukasika kuwanikwa pamwe nekudzivirirwa kweutachiona hweHIV hunokonzerwa neHPV ndiyo kiyi yekudzivirira gomarara remuchibereko.Izvo zvakakosha zvikuru kumisikidza yakapusa, yakananga uye nekukurumidza etiology diagnostic test yekiriniki yekuongororwa uye kurapwa kwegomarara rechibereko.
Channel
| Channel | Type |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Quasar 705/CY5.5 | Kudzora Kwemukati |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | Weti, Cervical Swab, Vaginal Swab |
| Ct | ≤28 |
| LoD | 300 Makopi/mL |
| Zvakananga | Hapana kuyambuka-reactivity nemamwe masampuli ekufema akadai seInfluenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. uye human genomic DNA. |
| Zvishandiso Zvishandiso | MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Chaiyo-Nguva PCR System uye BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
1.Weti muenzaniso
A: Tora1.4mL yemuenzaniso weti inoongororwa uye centrifuge pa 12000rpm kwemaminitsi mashanu;rasa iyo supernatant (zvinokurudzirwa kuchengetedza 10-20μL supernatant kubva pasi pe centrifuge chubhu), wedzera 200μL yemuenzaniso wekuburitsa reagent, uye iyo inotevera kudhirowa kunofanirwa kuitwa maererano nemirairo yekushandiswa kweMacro & Micro-Test Sample Release. Reagent (HWTS-3005-8).
B: Tora1.4mL yemuenzaniso weti inoongororwa uye centrifuge pa12,000rpm kwemaminitsi mashanu;bvisa supernatant (zvinokurudzirwa kuchengeta 10-20μL ye supernatant kubva pasi pe centrifuge chubhu), uye wedzera 200μL yesaline yakajairwa kuti umise zvakare, semuenzaniso unoongororwa.Iko kunotevera kubviswa kunogona kuitwa neMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) naJiangsu Macro & Micro-Test Med-Tech Co., Ltd.s zvekushandisa.Iyo yakakurudzirwa elution vhoriyamu i80μL.
C: Tora1.4mL yemuenzaniso weti inoongororwa uye centrifuge pa12,000rpm kwemaminitsi mashanu;bvisa supernatant (zvinokurudzirwa kuchengeta 10-20μL ye supernatant kubva pasi pe centrifuge chubhu), uye wedzera 200μL yesaline yakajairwa kuti umise zvakare, semuenzaniso unoongororwa.Iyo inotevera yekuwedzera inogona kuitwa neQIAamp DNA Mini Kit(51304) neQIAGEN kana Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Kubvisa kunofanira kugadziriswa maererano nemirairo yekushandisa.Iyo yekubvisa sampuli vhoriyamu ndeye 200μL, uye vhoriyamu yakakurudzirwa inosvika makumi masereμL.
2. Cervical swab / vaginal swab sample
A: Tora 1mL yemuenzaniso kuti uongororwe mu 1.5mLof centrifuge chubhu,uyecentrifuge pa12000rpm kwemaminitsi mashanu. Discard the supernatant (inokurudzirwa kuchengeta 10-20μL ye supernatant kubva pasi pe centrifuge chubhu), wedzera 100μL yemuenzaniso wekuburitsa reagent, wobva wabvisa zvinoenderana nemirairo yekushandiswa kweMacro & Micro-Test Sample Release Reagent ( HWTS-3005-8).
B: Kubvisa kunogona kuitwa neMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. zvakanyatsoenderana nemirairo yekushandiswa.Iyo yakabviswa sampuli vhoriyamu i200μL, uye yakakurudzirwa elution vhoriyamu i80μL.
C: Kubvisa kunogona kuitwa neQIAamp DNA Mini Kit (51304) neQIAGEN kana Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Kubvisa kunofanira kugadziriswa maererano nemirairo yekushandisa.Iyo yekubvisa sampuli vhoriyamu ndeye 200 μL, uye yakakurudzirwa elution vhoriyamu ndeye80 μL.
3, Cervical Swab/Vaginal Swab
Usati watora sampling, shandisa donje swab kupukuta zvinyoro nyoro zvakabuda kubva mumuromo wechibereko, uye shandisa imwe donje swab yakapinzwa nesero rekuchengetedza mhinduro kana cervical exfoliated cell sampling bhurashi kuti ubatirire kumuromo wekervical mucosa uye shandura clockwise 3-5 kutenderera kuti uwane. cervical exfoliated cells.Zvishoma nezvishoma tora donje swab kana bhurasho,uyeisa muchubhu yekuenzanisira ine 1mL yesaline isina utachiona. AKana wanyatsogeza, svina omesa donje swab kana bhurasho pamadziro echubhu worasa, sunga chubhu, womaka zita remuenzaniso (kana nhamba) wonyora pachubhu yekuenzanisira.
