Mhando gumi nemana dzezvirwere zvinotapukira zvakabatanidzwa
Zita rechigadzirwa
HWTS-RT159B Mhando gumi nemana dzezvirwere zvinofema Combined Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Utachiona hwemunzira yekufema ndicho chirwere chinonyanya kuwanikwa muvanhu, chinogona kuitika chero muvanhurume kana kuti mukadzi, zera uye nzvimbo ipi zvayo. Ndicho chimwe chezvikonzero zvikuru zvekurwara nekufa kwevanhu pasi rose.[1]. Zvirwere zvekufema zvinowanzo sanganisira coronavirus itsva, Furuwenza A, Furuwenza B, Furuwenza yekufema syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, uye Streptococcus pneumoniae, nezvimwewo.[2,3].
Chiteshi
| Nzvimbo yetsime | Zita reMhinduro yeKupindura | Utachiona Hunofanira Kuonekwa |
| 1 | Musanganiswa Mukuru A | SARS-CoV-2, IFV A, IFV B |
| 2 | Musanganiswa Mukuru B | Mumiririri, hMPV, MP, Cpn |
| 3 | Musanganiswa Mukuru C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Musanganiswa Mukuru D | CoV, EV, SP, kutonga kwemukati |
| 5 | Musanganiswa Mukuru A | SARS-CoV-2, IFV A, IFV B |
| 6 | Musanganiswa Mukuru B | Mumiririri, hMPV, MP, Cpn |
| 7 | Musanganiswa Mukuru C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Musanganiswa Mukuru D | CoV, EV, SP, kutonga kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | Chinobvisa dumbu mumuromo, chinobvisa dumbu mumhino |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Makopi/mL |
| Kunyatsojeka | Mhedzisiro yebvunzo ye cross-reactivity yakaratidza kuti pakanga pasina kusangana pakati pe kit iyi neCytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii uye human genomic nucleic acids. |
| Zvishandiso Zvinoshanda | Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.)Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi, Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Chinorayirwa chekuburitsa chinhu chinokurudzirwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Vhoriyamu yemuenzaniso yakabviswa i200µL. Matanho anotevera anofanira kuitwa zvichienderana nemirayiridzo yekushandisa chinobvumirwa ichi chekuburitsa chinhu. Vhoriyamu yekudzikisa inokurudzirwa ndeye80µL.
