Virusi reFivha yeHemorrhagic yeXinjiang
Zita rechigadzirwa
HWTS-FE007B/C Xinjiang Hemorrhagic Fever Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Virusi reXinjiang hemorrhagic fever rakatanga kutorwa kubva muropa revarwere vane hemorrhagic fever muTarim Basin, Xinjiang, China uye hard ticks dzakabatwa munharaunda, uye rakatumidzwa zita raro. Zviratidzo zvekiriniki zvinosanganisira fivha, musoro, kubuda ropa, hypotensive shock, nezvimwewo. Kuchinja kukuru kwechirwere ichi kunowedzera kuwanda kwemasero eropa, kuzara kweropa, kuwedzera kwemvura inoyerera uye kusasimba, zvichikonzera kuwanda kwakasiyana-siyana kweropa uye kubuda ropa muganda nemumukati pamwe chete nematishu enhengo dzakasiyana-siyana mumuviri wese, nekuora uye kupararira kwenhengo dzakasimba dzakadai sechiropa, adrenal gland, pituitary gland, nezvimwewo, uye kuzvimba kwakaita sejelly mu retroperitoneum.
Chiteshi
| FAM | Virusi reFivha yeHemorrhagic yeXinjiang |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | seramu itsva |
| Tt | ≤38 |
| CV | ＜5.0% |
| LoD | Makopi 1000/mL |
| Kunyatsojeka | Hapana kusangana kwezvirwere zvakasiyana-siyana pamwe chete nezvimwe zviratidzo zvekufema zvakaita seInfluenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, nezvimwewo uye human genomic DNA. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu 7500 Masisitimu EPCR Enguva Yechokwadi, Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Chinorayirwa chekuburitsa chinhu: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuburitsa chinhu kunofanirwa kuitwa zvichienderana nemirairo yekushandisa chinobvumirwa ichi chekuburitsa chinhu. Vhoriyamu yemuenzaniso inobviswa i200µL, uye vhoriyamu yekudzikisa chinhu i80µL.
Chinorayirwa kubvisa: QIAamp Viral RNA Mini Kit (52904) neQIAGEN neNucleic Acid Extraction kana Purification Reagent (YDP315-R). Kubvisa kunofanirwa kuitwa zvichienderana nemirairo yekushandisa. Vhoriyamu yemuenzaniso yakabviswa i140µL, uye vhoriyamu yekudzikisa inokurudzirwa i60µL.
