Mhando nhanhatu dzezvirwere zvekufema
Zita rechigadzirwa
HWTS-OT058A/B/C/Z-Kit yeRT-PCR inopenya panguva chaiyo yekuona mhando nhanhatu dzezvirwere zvinotapukira
Chitupa
CE
Epidemiology
Chirwere cheCoronavirus Virus 2019, chinonzi "COVID-19", chinoreva pneumonia inokonzerwa nehutachiona hweSARS-CoV-2. SARS-CoV-2 chirwere chekoronavirus chiri murudzi rweβ. COVID-19 chirwere chinotapukira chinopararira nekukurumidza, uye vanhu vanowanzo kubatwa nechirwere ichi. Parizvino, chirwere ichi chinowanzo konzerwa nehutachiona hweSARS-CoV-2, uye vanhu vasina zviratidzo vanogonawo kuva ivo vanotapukirwa. Zvichienderana nekuferefeta kuri kuitwa pari zvino, nguva yekuzvitakura imazuva 1-14, kazhinji mazuva 3-7. Fivha, kukosora kwakaoma uye kuneta ndizvo zviratidzo zvikuru. Varwere vashoma vaive nemhino dzakavharika, mhino ichiyerera, pahuro panorwadza, myalgia uye manyoka.
Furuwenza, inozivikanwa se "furuwenza", chirwere chinotapukira chekufema chinokonzerwa nevhairasi yefuruwenza. Chinotapukira zvakanyanya. Chinonyanya kutapuriranwa nekukosora nekuhotsira. Chinowanzo tanga muchirimo nemuchando. Mavhairasi efuruwenza akakamurwa kuita furuwenza A, IFV A, furuwenza B, IFV B, uye Furuwenza C, IFV C mhando nhatu, dzese dziri dzevhairasi inonamira, inokonzera chirwere chevanhu kunyanya mavhairasi efuruwenza A neB, ivhairasi yeRNA ine tambo imwe chete, yakapatsanurwa. Vairasi yefuruwenza A ivhairasi yekufema inopinza, inosanganisira H1N1, H3N2 nedzimwe mhando, dzinokurumidza kuchinja uye kupararira pasi rose. "Shift" inoreva kuchinja kwevhairasi yefuruwenza A, zvichikonzera kubuda kwevhairasi itsva "subtype". Mavhairasi efuruwenza B akakamurwa kuita madzinza maviri, Yamagata neVictoria. Vairasi yefuruwenza B ine antigenic drift chete, uye inonzvenga kutarisa nekubviswa kwemasoja emuviri evanhu kuburikidza nekuchinja kwayo. Zvisinei, kumhanya kwevhairasi yefuruwenza B kunononoka kupfuura kwevhairasi yefuruwenza A yevanhu. Utachiona hwefuruwenza B hunogonawo kukonzera hutachiona hwekufema muvanhu uye hunokonzera zvirwere.
Adenovirus (AdV) ndeye adenovirus yemhuka dzinoyamwisa, inova hutachiona hweDNA hwakakamurwa kaviri husina kuvharwa. Kune marudzi angangoita makumi mapfumbamwe emasero akawanikwa, ayo anogona kukamurwa kuita magenera eAG 7. Utachiona hweAdV hunogona kukonzera zvirwere zvakasiyana-siyana, zvinosanganisira pneumonia, bronchitis, cystitis, eye conjunctivitis, zvirwere zvemudumbu uye encephalitis. Adenovirus pneumonia ndeimwe yemhando dzakakomba dzepneumonia inowanikwa munharaunda muvana, ichikonzera inenge 4%-10% yepneumonia inowanikwa munharaunda.
Mycoplasma pneumoniae (MP) rudzi rwehutachiona hudiki hweprokaryotic, huri pakati pebhakitiriya nehutachiona, hune chimiro chemasero asi husina madziro emasero. MP inonyanya kukonzera hutachiona hwekufema kwevanhu, kunyanya kuvana nevechidiki. Hunogona kukonzera mycoplasma pneumonia yevanhu, hutachiona hwekufema kwevana uye pneumonia isingawanzoitiki. Zviratidzo zvekiriniki zvakasiyana, zvakawanda zvacho kukosora kwakanyanya, fivha, dzihwa, musoro, kurwadziwa pahuro. Utachiona hwekufema hwepamusoro uye pneumonia yebronchial ndizvo zvinonyanya kuitika. Vamwe varwere vanogona kukura kubva pahutachiona hwekufema hwepamusoro kusvika papneumonia yakanyanya, kufema kwakanyanya uye rufu runogona kuitika.
Virusi reRespiratory syncytial virus (RSV) ivhairasi yeRNA, iri mumhuri yeparamyxoviridae. Inotapuriranwa nemadonhwe emhepo uye kusangana kwepedyo uye ndiyo hosha huru yehutachiona hwepazasi pekufema muvacheche. Vacheche vane hutachiona hweRSV vanogona kuva nebronchiolitis yakakomba (inonzi bronchiolitis) uye pneumonia, iyo ine chekuita neasthma muvana. Vacheche vane zviratidzo zvakakomba, zvinosanganisira fivha yakanyanya, rhinitis, pharyngitis uye laryngitis, uyezve bronchiolitis uye pneumonia. Vana vashoma vanorwara vanogona kuoma ne otitis media, pleurisy uye myocarditis, nezvimwewo. Utachiona hwepazasi pekufema ndicho chiratidzo chikuru chehutachiona muvakuru nevana vakuru.
Chiteshi
| Zita rechiteshi | R6 Reaction Buffer A | R6 Reaction Buffer B |
| FAM | SARS-CoV-2 | HAdV |
| VIC/HEX | Kudzora Kwemukati | Kudzora Kwemukati |
| CY5 | IFV A | MP |
| ROX | IFV B | RSV |
Magadzirirwo ehunyanzvi
| Kuchengetera | Mvura: ≤-18℃ Murima; Yakapfava: ≤30℃ Murima |
| Nguva yekuzorora | Mvura: mwedzi 9; Lyophilized: mwedzi 12 |
| Rudzi rwemuenzaniso | Ropa rose, Plasma, Serum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Makopi 300/mL |
| Kunyatsojeka | Mhedzisiro yekubatana kwemasero yakaratidza kuti pakanga pasina kusangana pakati pekiti ne coronavirus yevanhu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s. pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci and newborn cryptococcus and human genomic nucleic acid. |
| Zvishandiso Zvinoshanda | Inogona kuenzana nemidziyo yePCR inopenya iri pamusika.Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Masisitimu eABI 7500 PCR Enguva Yechokwadi Masisitimu ePCR eABI 7500 Anokurumidza Kushanda Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Chiedza cheKufambisa Mwenje®Masisitimu ePCR e480 Enguva Yechokwadi Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi, BioRad Sisitimu yePCR yeCFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
