Utachiona hwekufema hwakabatanidzwa
Zita rechigadzirwa
HWTS-RT183-Chigadzirwa Chekuona Zvirwere Zvinofema Zvakasanganiswa (Fluorescence PCR)
Epidemiology
Chirwere cheCoronavirus Virus 2019, chinonzi 'COVID-19', chinoreva pneumonia inokonzerwa nehutachiona hweSARS-CoV-2. SARS-CoV-2 chirwere chekoronavirus chiri murudzi rwe β. COVID-19 chirwere chinotapukira chekufema zvakanyanya, uye vanhu vanowanzo kubatwa nechirwere ichi. Parizvino, chirwere ichi chinowanzo batwa nechirwere ichi ne2019-nCoV, uye vanhu vasina zviratidzo vanogonawo kuva ivo vanobatwa nechirwere ichi. Zvichienderana nekuferefeta kuri kuitwa pari zvino, nguva yekuzvitakura imazuva 1-14, kazhinji mazuva 3-7. Fivha, kukosora kwakaoma uye kuneta ndizvo zviratidzo zvikuru. Varwere vashoma vaiva nezviratidzo zvakaita sekuzara kwemhino, mhino inoyerera, pahuro panorwadza, myalgia uye manyoka, nezvimwewo. Furuwenza, inozivikanwa se 'furuwenza', chirwere chinotapukira chekufema zvakanyanya chinokonzerwa nehutachiona hwefuruwenza. Chinotapukira zvakanyanya. Chinonyanya kutapuriranwa nekukosora nekuhotsira. Chinowanzo tanga muchirimo nemuchando. Mavhairasi efuruwenza akakamurwa kuita mhando nhatu dzefuruwenza A (IFV A), furuwenza B (IFV B), uye furuwenza C (IFV C), ese ari evhairasi inonamira, anokonzera chirwere chevanhu kunyanya mavhairasi efuruwenza A neB, ivhairasi yeRNA ine tambo imwe chete. Vhairasi yefuruwenza A ivhairasi inofema zvakanyanya, inosanganisira H1N1, H3N2 nedzimwe mhando, dzinokurumidza kuchinja uye kupararira pasi rose. 'Shift' inoreva kuchinja kwevhairasi yefuruwenza A, zvichikonzera kubuda kwevhairasi itsva 'subtype'. Mavhairasi efuruwenza B akakamurwa kuita madzinza maviri, vhairasi yeYamagata neVictoria Influenza B ine antigen drift chete, uye inodzivisa kutariswa nekubviswa kwemasoja emuviri wemunhu kuburikidza nekuchinja kwayo. Zvisinei, kumhanya kwevhairasi yefuruwenza B kunononoka kupfuura kwevhairasi yefuruwenza A yevanhu. Vhairasi yefuruwenza B inogonawo kukonzera hutachiona hwekufema kwevanhu uye kutungamira kuzvirwere zvinopararira.
Virusi reRespiratory syncytial virus (RSV) ivhairasi yeRNA, iri mumhuri yeparamyxoviridae. Inotapuriranwa nemadonhwe emhepo uye kusangana kwepedyo uye ndiyo hosha huru yehutachiona hwepazasi pekufema muvacheche. Vacheche vane hutachiona hweRSV vanogona kukura nebronchiolitis uye pneumonia, izvo zvine chekuita neasthma muvana. Vacheche vane zviratidzo zvakakomba, zvinosanganisira fivha yakanyanya, rhinitis, pharyngitis uye laryngitis, uyezve bronchiolitis uye pneumonia. Vana vashoma vanorwara vanogona kuoma ne otitis media, pleurisy uye myocarditis, nezvimwewo. Utachiona hwepazasi pekufema ndicho chiratidzo chikuru chehutachiona muvakuru nevana vakuru.
Magadzirirwo ehunyanzvi
| Kuchengetera | -18℃ Murima |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | swab yemumuromo; swab yemumhino |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | Makopi mazana maviri/mL |
| Kunyatsojeka | Mhedzisiro yekuchinjana kwemasero inoratidza kuti hapana kusangana pakati pekiti ne cytomegalovirus, herpes simplex virus type 1, varicella zoster virus, Epstein-Barr virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus type I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Bordetella pertussis, Corynebacterium spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp., Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcescens, Citrobacter rodentium, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q fever uye human genomic nucleic acid. |
| Zvishandiso Zvinoshanda | Maitiro ekushandisa eApplied Biosystems 7500 Masisitimu ePCR eNguva Yechokwadi, Maitiro ekushandisa eApplied Biosystems 7500 Masisitimu ePCR eNguva Yechokwadi, QuantStudio®Masisitimu mashanu ePCR Enguva Yechokwadi, SLAN-96P Masisitimu ePCR Enguva Yechokwadi (Hongshi Medical Technology Co., Ltd.), LightCycler®Sisitimu yePCR ye480 Real-Time, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, tekinoroji yeHangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. |
Kufamba Kwebasa
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), uye Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (inogona kushandiswa neEudemonTM AIO800 (HWTS-EQ007)) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Vhoriyamu yemuenzaniso yakatorwa i200μL uye vhoriyamu yekudzikisa inokurudzirwa i150μL.
