Orientia tsutsugamushi
Zita rechigadzirwa
HWTS-OT002B Orientia tsutsugamushiKiti Yekuona Nucleic Acid (Fluorescence PCR)
Epidemiology
Scrub typhus chirwere chinokonzerwa nechirwere cheOrientia tsutsugamushi (Ot). Orientia scrub typhus ibhakitiriya rine utachiona hweGram-negative rinopinda mumasero. Orientia scrub typhus iri muboka reOrientia muchikamu cheRickettsiales, mhuri yeRickettsiaceae, uye rudzi rweOrientia. Scrub typhus inonyanya kutapuriranwa nekurumwa nemakonye ane utachiona. Inoratidzwa nefivha yakakwira, eschar, lymphadenopathy, hepatosplenomegaly, uye peripheral blood leukopenia, nezvimwewo. Muzviitiko zvakakomba, inogona kukonzera meningitis, kutadza kushanda kwechiropa neitsvo, kutadza kushanda kwenhengo dzakawanda dzemuviri, uye kunyange kufa.
Chiteshi
| FAM | Orientia tsutsugamushi |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | seramu itsva |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/μL |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi, Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Zvinokurudzirwa kuti munhu abvise reagent: Macro & Micro-TestMukuruKiti yeDNA/RNA (HWTS-301)9) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubviswa kwemvura kunofanirwa kuitwa zvichienderana nemirairo yekushandisa reagent iyi yekuburitsa. Vhoriyamu yemuenzaniso yakabviswa i200µL, uye vhoriyamu yekudzikisa mvura inokurudzirwa.100µL.
