EasyAmp neMacro & Micro Test—-Chishandiso Chinotakurika Chekuwedzera Kupenya Kwechiedza Chinoenderana neLAMP/RPA/NASBA/HDA

Kushanda Kwakanaka & Kushandiswa Kwakakura

Easy Amp, kuburikidza netekinoroji ye ikuwedzera kwe nucleic acid mumhepo inopisainoratidzwa nehukurukunzwa uyepfupimaitironguva isina zvinodiwa zve maitiro ekuchinja tembiricha. Naizvozvo, inekubudaedsevazhinjitekinoroji yaifarirwa zvikuru yekuyedza nucleic acid nekukurumidza.Pasina kuomararamasisitimu ekufambisa mabhasikoro emishonga, chishandiso chiri nyore uye chinotakurika chekuwedzera simba remuvirizvinogona kugutsamaitiro ese ekuita.

Macro & Micro-Test yakagadzira yakazvimirirandokutangaiyo EasyAmp,chishandiso chinotakurika chekuwedzera fluorescent isothermalzvekushandisa zvakasiyana-siyana.

Parizvino,Amp iri nyore yakasimbiswa kutiInoenderana nenzira dzakasiyana-siyana dzekuwedzera simba remuviri: Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification (NASBA), uye Helicase Dependent Amplification (HDA)Ichava imwe nzira yemazuva ano yekuedza nucleic acid nekukurumidza uye kushandiswa kwakakura.

· Kuwedzeredzwa kweIsothermal kwakabatana neLoop (RAMP):Kuwedzera simba pa60-65°Cnezvosemaitiromaitirozvapera mukati memaminitsi makumi matatu.Kuona Mufananidzo 1 wekuwedzera kwayomusimboti:

 

Mufananidzo 1. Nheyoof MWENJO 

· Kuwedzerwa kweRecombinase Polymerase (RPA/RAA/MIRA):Kuwedzera simba pa37°C-42°Cnezvosemaitiromaitirozvinopera mumaminitsi gumi kusvika makumi matatu.Kuona Mufananidzo 2 wemusimboti wekuwedzera simba:

Mufananidzo 2. Nheyoof Kuwedzera kwePolymerase Recombinase

·Kuwedzerwa kweNucleic Acid Sequence Based Amplification (NASBA):Kuwedzera simba pa42°Cnezvosemaitiromaitirozvinopera mumaminitsi 40-120.Kuona Mufananidzo 3 wemusimboti wekuwedzera simba:

Mufananidzo 3. Nheyoof Kuwedzera kweNucleic Acid Sequence

· Kuwedzeredzwa Kwesimba reHelicase (HDA):Kuwedzera simba pa65°Cnezvosemaitiromaitirozvinopera mumaminitsi 70-90.Kuona Mufananidzo 4 wemusimboti wekuwedzera simba:

Mufananidzo 4. Nheyoof Kuwedzerwa Kwesimba reHelicase

· Iyo EasyAmpgonesaKuwedzerwa kweDNA neRNA kunoshanda uye kwakasimba uchishandisa nzira ina dze isothermal dzataurwa pamusoro apa. Kunoita kuti amplification isvike pamakopi mazana maviri pa mL uye kunoita kuti pave nekujeka kwakanyanya pasina cross-reactivity mu target amplification..(Iinoratidzwa muMifananidzo 5 kusvika ku7).

Kuwanikwa kweDNA Kuwanikwa kweRNA

Mufananidzo 5. Puratifomu yeRAA/MIRA

Kuwanikwa kweDNA Kuwanikwa kweRNA

Mufananidzo 6. Puratifomu yeMwenje

 

Kuwanikwa kweDNA Kuwanikwa kweRNA

Mufananidzo 7. Puratifomu yeNASBA

Zvakanakira Chigadzirwa:

Kukurumidza

· EPIAtekinoroji inoitamhedzisiro yakanakayakawanikwa mumaminitsi mashanu.

· Kushanda kuri nyore nekubvisa nhanho imwe cheteyekuwedzera simba pachiridzwa.

Inoshanda zvakanaka

· Mamodule mana akazvimiririra ekuyedza panguva imwe chete e4 akasiyana panguva imwe chetemhando yekuedzas.

· Module imwe neimwe inokwanisa kuongorora mienzaniso mina panguva imwe chete.

· Dual/Quadfluorescencenzirakuti zvionekwe zvakanyanya nevanhu vakawanda.

Kuenderana

· Inoenderana neakawandamapuratifomu e isothermal akadai seLAMP, RPA, NASBA, HDA.

· Yakagadzirirwa kunyorwa kwereverse transcription mushure mekudziya kwakanyanya.

· Gadzirisakugonakusangana nezvinodiwa nevatengi.

Zviri nyore kushandisa

· Pdhizaini yetafura(290×245×128mm, 2kg) ye masenarios akasiyana-siyana ekushandisa.

· Chinobatidza chekubata chine mavara e7-inchkuti zvive nyore kushanda uye kuti zvive nemifananidzo yakajeka yemhedzisiro.

· Printer yekupisa yakavakirwa mukati kuti ibudise mhinduro ipapo ipapo.

· Yakabatanidzwa scanner yekuverenga otomatiki zvinhu.