MTHFR Gene Polymorphic Nucleic Acid
Zita rechigadzirwa
Kiti yeHWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)
Epidemiology
Folic acid ivhitamini inonyungudika mumvura iyo inokosha mukushanda kwemuviri. Mumakore achangopfuura, zvidzidzo zvakawanda zvakaratidza kuti, kuchinja kwe folate metabolizing enzyme gene MTHFR kuchakonzera kushomeka kwe folic acid mumuviri, uye kukuvara kunowanzoitika kwe folic acid kushomeka muvanhu vakuru kunogona kukonzera megaloblastic anemia, vascular endothelial damage, nezvimwewo. Kushomeka kwe folic acid mumadzimai ane pamuviri hakugone kugutsa zvinodiwa zvavo nemwana ari mudumbu, izvo zvinogona kukonzera neural tube defects, anencephaly, stillbirth, uye miscarriage. Mazinga e serum folate anokanganiswa ne 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms. Kuchinja kwe 677C>T uye 1298A>C mu MTHFR gene kunokonzera kushandurwa kwe alanine kuita valine ne glutamic acid, zvichiteerana, zvichikonzera kudzikira kweMTHFR activity uye nekudaro kudzikira kwekushandiswa kwe folic acid.
Chiteshi
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC(HEX) | Kudzora kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Ropa richangobva kuunganidzwa reEDTA risingaunganidzi ropa |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Zvishandiso Zvinoshanda: | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eSLAN ®-96P Enguva Yechokwadi Masisitimu mashanu ePCR eQuantStudio™ Munguva Yechokwadi Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1
Zvinokurudzirwa kuti zvibudiswe: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Sarudzo 2
Zvinokurudzirwa kuti zvibviswe: Blood Genomic DNA Extraction Kit (YDP348, JCXB20210062) naTiangen Biotech (Beijing) Co., Ltd. Blood Genome Extraction Kit (A1120) naPromega.
