Kunyora kwehutachiona hweMonkeypox Nucleic Acid
Zita rechigadzirwa
HWTS-OT201Kiti yeKuona Kunyorwa kweNyukireya yeMonkeypox Virus(Fluorescence PCR)
Epidemiology
Monkeypox (Mpox) chirwere chinotapukira chinokonzerwa neMonkeypox Virus (MPXV). MPXV ine chimiro chedenderedzwa kana kuti denderedzwa, uye ihutachiona hweDNA hune tambo mbiri hune hurefu hweinenge 197Kb. Chirwere ichi chinonyanya kutapurirwa nemhuka, uye vanhu vanogona kutapukirwa nekurumwa nemhuka dzine utachiona kana nekubata zvakananga neropa, mvura yemuviri uye mapundu emhuka dzine utachiona. Hutachiona hunogonawo kutapurirwa pakati pevanhu, kunyanya kuburikidza nemadonhwe ekufema panguva yekusangana zvakananga kana kubata zvakananga nemvura yemuviri wemurwere kana zvinhu zvakasvibiswa. Zvidzidzo zvakaratidza kuti MPXV inoumba clades mbiri dzakasiyana: clade I (yaimbozivikanwa seCentral African clade kana Congo Basin clade) uye clade II (yaimbonzi West African clade). Mpox yeCongo Basin clade yakaratidzwa zvakajeka kuti inotapuriranwa pakati pevanhu uye inogona kukonzera rufu, nepo mpox yeWest African clade ichikonzera zviratidzo zvishoma uye ine mwero wakaderera wekutapurirana kubva kuvanhu kuenda kuvanhu.
Mhedzisiro yebvunzo iyi haisi iyo chete inoratidza kuongororwa kwehutachiona hweMPXV muvarwere, iyo inofanirwa kubatanidzwa nehunhu hwemurwere pamwe nedzimwe data rebvunzo yerabhoritari kuti vaone nemazvo hutachiona hwehutachiona uye kugadzira hurongwa hwekurapa hwakakodzera kuti vave nechokwadi chekuti kurapwa kwakachengeteka uye kunoshanda.
Magadzirirwo ehunyanzvi
| Rudzi rwemuenzaniso | mvura yemapundu emunhu, swab yemuromo uye serum |
| Ct | 38 |
| FAM | chikamu cheFAM-MPXV II chikamu cheVIC/HEX-MPXV I |
| CV | ≤5.0% |
| LoD | Makopi mazana maviri/μL |
| Kunyatsojeka | Shandisa kit iyi kuona mamwe mavhairasi, akadai seSmallpox virus, Cowpox virus, Vaccinia virus, HSV1, HSV2, Herpesvirus yemunhu rudzi rwechitanhatu, Herpesvirus yemunhu rudzi rwechinomwe, Herpesvirus yemunhu mhando 8, Measels vieus, Chicken pox-Herpes zoster virus, EB virus, Rubella virus nezvimwewo, uye hapana kusangana kwevamwe. |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo BioRad CFX96 Real-Time PCR Systems BioRad CFX Opus 96 Masisitimu ePCR Enguva Yechokwadi |
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