Middle East Respiratory Syndrome Coronavirus Nucleic Acid
Zita rechigadzirwa
HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit (Fluorescent PCR)
Epidemiology
Middle East respiratory syndrome coronavirus (MERS-CoV), β-coronavirus inokonzerachirwere chekufema muvanhu, chakatanga kuonekwa mumurwere wechirume wekuSaudi Arabia ane makore makumi matanhatu okuberekwa akafa musi wa24 Chikunguru, 2012. Kuonekwa kwechirwere cheMERS-CoV kunobva pakusava nezviratidzo kana zviratidzo zvekufema zvishoma kusvika kuchirwere chekufema chakanyanya kusvika parufu.
Chiteshi
| FAM | RNA yehutachiona hweMERS |
| VIC(HEX) | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ Murima |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | Ma swabs emumhino achangobva kuunganidzwa |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | Makopi 1000/mL |
| Kunyatsojeka | Hapana kusangana kwezvirwere zvinotapukira zvinosangana nehutachiona hwecoronavirus yevanhu. |
| Zvishandiso Zvinoshanda: | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
Chinorayirwa kubvisa: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction kana Purification Reagent (YDP315-R) naTiangen Biotech (Beijing) Co., Ltd.
Sarudzo yechipiri.
Chinoshandiswa pakuburitsa zvinhu chinokurudzirwa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Zvikamu zvezvigadzirwa
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Mhando gumi nemapfumbamwe dzeNucleic Acid yeKufema
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Antigen yehutachiona hwekufema hweSyncytial
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Mhando nhanhatu dzezvirwere zvekufema
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AdV Universal uye Type 41 Nucleic Acid
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Furuwenza A Virus Nucleic Acid
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Furuwenza A/B Antigen
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Furuwenza B Virus Nucleic Acid
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Adenovirus Antigen
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Mycoplasma Pneumoniae Nucleic Acid