Kiti yeKuona Legionella Pneumophila Nucleic Acid
Zita rechigadzirwa
Kiti yeHWTS-RT163-Legionella Pneumophila Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Legionella pneumophila ibhakitiriya remhando yeflagellated, gram-negative, short coccobacillus yerudzi rweLegionella polymorphic. Legionella pneumophila ibhakitiriya refacultative parasitic rinogona kupinda mu amoeba kana macrophages evanhu. Kutapukira kwebhakitiriya iri kunowedzera zvikuru kana paine ma antibodies uye serum complement (asi kuvapo kwezvose hazvidiwi zvachose). Legionella pneumophila ibhakitiriya rakakosha rinokonzera denda uye sporadic community-acquired pneumonia uye hospital-acquired pneumonia, zvichiita kuti pave ne80% yeLegionella pneumonia. Legionella pneumophila inonyanya kuwanikwa mumvura nevhu. Kutorwa kwemvura nevhu zvakasvibiswa mumuviri wemunhu nenzira yeaerosol kungave ndiyo nzira huru yehutachiona hweLegionella. Parizvino, nzira huru dzekuona nekuongorora Legionella pneumophila murabhoritari ibhakitiriya culture uye serological assay.
Magadzirirwo ehunyanzvi
| Kuchengetera | -18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | pfapfaidzo |
| Ct | ≤38 |
| CV | 5.0% |
| LoD | Makopi 1000/μL |
| Zvishandiso Zvinoshanda | Inoshanda kune reagent yekuona type I: Applied Biosystems 7500 Real-Time PCR Systems,QuantStudio®5 Real-Time PCR Systems,SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.),LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology),MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.),BioRad CFX96 Real-Time PCR System,BioRad CFX Opus 96 Real-Time PCR System. |
| Inoshanda kune reagent yekuona mhando yechipiri: EudemonTM AIO800 (HWTS-EQ007) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Kufamba Kwebasa
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), uye Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (inogona kushandiswa neEudemonTM AIO800 (HWTS-EQ007)) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Vhoriyamu yemuenzaniso yakatorwa i200μL uye vhoriyamu yekudzikisa inokurudzirwa i150μL.
