Klebiela pneumoniae, acinostobager
Chigadzirwa Zita
HWTS-RT109 Kneumoniae, Acinetabactter Baumannii And Pseudomonas Aeruginosa uye Drug, Ndim, Oxa48 uye Importescence PCRT)
Chitupa
CE
Epidemiology
Klebiela pneumoniae icliniki yakajairika patiistic uye imwe yeakakosha pathogenic bactia inokonzeresa hutachiona. Kana kupokana kwemuviri kukaderedzwa, mabhakitiriya anopinda mumapapu kubva patsvanyiriri hwekufema, zvichikonzera hutachiona mune dzakawanda nhengo dzemuviri, uye kushandiswa kwekutanga kwemishonga inorwisa mabhakitiriya kurapa[1].
Nzvimbo inozivikanwa kwazvo yeAcinetabactter Baumannii kutapuranga ndiyo mapapu, iyo yakakosha pathogen yechipatara yakawanikwa pneumonia (hap), kunyanya ventilator yakabatana pneumonia (VAP). Inowanzo kuperekedzwa nedzimwe hutachiona uye fungul hutachiona, nehunhu hwepamusoro morbidity rate uye huwandu hwakakura hwekufa.
Pseudomson AeruginosA ndiyo isinganyanyo-chicompative chirwere chekiriniki, uye ine mikana yakakosha yechipatara, nehunhu hwepanyama, kusiyana-siyana kusiyana uye kuwanda-zvinodhaka.
Chiteshi
Zita | PCR-Sanganisa 1 | PCR-Sanganisa 2 |
Fam chiteshi | Aba | Imp |
Vic / Hex chiteshi | Kutonga kwemukati | KPC |
Cy5 Channel | PA | Ndim |
Rox Channel | KPN | Oxa48 |
Technical parameter
Chengetedza | ≤-18 ℃ |
Shefu-Hupenyu | Mwedzi gumi nemaviri |
Type Type | Sputum |
Ct | ≤36 |
CV | ≤10.0% |
Lod | 1000 CFU / ML |
Chokwadi | a) Muchinjiko-wekutarisisa bvunzo inoratidza kuti kit iyi yekuvhiringidza pamwe nedzimwe nzira dzekufema, dzakabatana. pneumoophia, escherichia coli, pseudomson fluescens, candida albicans, chlamydia pneumoniae, dentiratory adyovirus, enteroccucus uye sputum samples pasina zvinangwa, nezvimwe. b) kugona kwekunyepedzera: sarudza mucin, minocycline, Gentamicin, clindamycin, levoploxcin hydrochloride, ciprolancin, uye roxitharomycin, uye mhedzisiro yacho inoratidza kuti zvinhu zvekupindira zvataurwa pamusoro apa Usakanganise nekuonekwa kwe Klabsiella pneumoniae, acinostobager |
Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PCR Kuongororwa System (FQD-96A, Bioer Technology) Ma-6000 chaiyo-nguva quolitative thermal cyler (Suzhou molarray co., Ltd.) Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |