Klebsiella Pneumoniae, Acinetobacter Baumannii uye Pseudomonas Aeruginosa uye Drug Resistance Genes (KPC, NDM, OXA48 uye IMP) Multiplex

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Kit iyi inoshandiswa pakuongorora Klebsiella pneumoniae (KPN), Acinetobacter baumannii (Aba), Pseudomonas aeruginosa (PA) uye majini mana ekudzivirira carbapenem (anosanganisira KPC, NDM, OXA48 uye IMP) mumasampuli emapute emunhu, kupa hwaro hwenhungamiro yekuongorora kliniki, kurapwa uye mishonga kune varwere vanofungidzirwa kuti vane hutachiona hwebhakitiriya.

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HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii uye Pseudomonas Aeruginosa uye Drug Resistance Genes (KPC, NDM, OXA48 uye IMP) Multiplex Detection Kit (Fluorescence PCR)

Chitupa

Epidemiology

Klebsiella pneumoniae chirwere chinowanzo wanikwa pakurapa uye chimwe chezvirwere zvikuru zvinokonzera hutachiona hwepasocomial. Kana simba remuviri rikaderera, mabhakitiriya anopinda mumapapu achibva mudumbu, zvichikonzera hutachiona muzvikamu zvakawanda zvemuviri, uye kushandiswa kwekutanga kwemishonga inorwisa mabhakitiriya ndiyo kiyi yekurapa.^[1].

Nzvimbo inonyanya kubatwa nehutachiona hweAcinetobacter baumannii imapapu, inova chirwere chakakosha cheHospital acquired pneumonia (HAP), kunyanya Ventilator associated pneumonia (VAP). Inowanzo perekedzwa nedzimwe hutachiona hwemabhakitiriya nefungus, hune hunhu hwehuwandu hwezvirwere zvakanyanya uye huwandu hwerufu hwakawanda.

Pseudomonas aeruginosa ndiyo bacilli isingavirisi gram-negative inonyanya kuwanikwa mukiriniki, uye ibhakitiriya rinokosha rinopa mukana wekutapukirwa nehutachiona hunowanikwa muchipatara, hune hunhu hwekutapukirwa nyore nyore, kuchinja-chinja kuri nyore uye kusakwanisa kushandisa mishonga yakawanda.

Chiteshi

Zita	PCR-Musanganiswa 1	PCR-Musanganiswa 2
Nzira yeFAM	Aba	IMP
Mugero weVIC/HEX	Kudzora Kwemukati	KPC
CY5 Channel	PA	NDM
ROX Channel	KPN	OXA48

Magadzirirwo ehunyanzvi

Kuchengetera	≤-18℃
Nguva yekuzorora	mwedzi gumi nemiviri
Rudzi rwemuenzaniso	Masvoto
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/mL
Kunyatsojeka	a)Kuongororwa kwekuongorora kwehutachiona kunoratidza kuti kit iyi haina hutachiona hwehutachiona hwehutachiona hwekufema hwakafanana nedzimwe hutachiona hwekufema, dzakadai seStreptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus uye sputum samples dzisina zvinangwa, nezvimwewo. b)Kugona Kudzivisa Kukanganiswa: Sarudza mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, uye roxithromycin, nezvimwewo. kuti uedze kukanganiswa, uye mhedzisiro inoratidza kuti zvinhu zvinokanganisa zvataurwa pamusoro hazvikanganisi kuonekwa kweKlebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa uye carbapenem resistance genes KPC, NDM, OXA48 uye IMP.
Zvishandiso Zvinoshanda	Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio^®Masisitimu mashanu ePCR enguva chaiyo Chiedza cheKufambisa Mwenje^®Sisitimu yePCR ye480 Real-Time Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeBioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi