Virusi reFuruwenza A/ Virusi reFuruwenza B
Zita rechigadzirwa
Kiti yeHWTS-RT174-Influenza A Virus/Influenza B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Zvichibva pamusiyano weantigenic uripo pakati pegene reNP negene reM, mavhairasi efuruwenza anogona kukamurwa kuita mhando ina: vhairasi yefuruwenza A (IFV A), vhairasi yefuruwenza B (IFV B), vhairasi yefuruwenza C (IFV C) uye vhairasi yefuruwenza D (IFV D).[1]. Virusi reFuruwenza A rine marudzi akawanda ezvirwere uye ma serotypes akaomarara, uye rinogona kuwana kugona kupararira kune vanhu kuburikidza nekuchinja kwemajini uye adaptive mutations. Vanhu havana dziviriro inogara kwenguva refu kuvhairasi reFuruwenza A, saka vanhu vemazera ese vanowanzo kubatwa nechirwere ichi. Virusi reFuruwenza A ndiro hosha huru inokonzera denda reFuruwenza[2]. Utachiona hwefuruwenza B hunonyanya kuwanikwa munzvimbo diki uye parizvino hauna mhando dzakasiyana. Hutachiona hukuru hunokonzera hutachiona muvanhu ndehweB/Yamagata lineage kana kuti B/Victoria lineage. Pakati pezviitiko zvakasimbiswa zvefuruwenza munyika gumi neshanu dziri mudunhu reAsia-Pacific mwedzi wega wega, huwandu hwakasimbiswa hwefuruwenza B huri pakati pe0-92%.[3]Kusiyana nehutachiona hwefuruwenza A, mapoka chaiwo akadai sevana nevakwegura anogona kubatwa nehutachiona hwefuruwenza B uye anowanzosangana nematambudziko, izvo zvinoremedza nzanga kupfuura hutachiona hwefuruwenza A.[4].
Chiteshi
| FAM | asidhi ye nucleic yeMP |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Muenzaniso we swab yemumuromo |
| Ct | Furuwenza A, Furuwenza BCt≤35 |
| CV | <5.0% |
| LoD | Furuwenza A neFuruwenza Bese ari 200Copies/mL |
| Kunyatsojeka | Kupindirana kwemasero: Hapana kusangana pakati pekiti neBocavirus, rhinovirus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, Epstein-Barr virus, herpes simplex virus, Varicella-zoster virus, mumps virus, enterovirus, measles virus, human metapneumovirus, adenovirus, human coronavirus, novel coronavirus, SARS coronavirus, MERS coronavirus, rotavirus, norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, uye DNA ye genomic yemunhu. Kuongororwa kwemushonga: Sarudza mucin (60 mg/mL), ropa revanhu (50%), phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (20mg/mL) ine 5% preservative, beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L) yekuedzwa kwekupindira, uye mhedzisiro yacho inoratidza kuti zvinhu zvinopindira pahuwandu hwataurwa pamusoro apa hazvikanganisi kuonekwa kwekit. |
| Zvishandiso Zvinoshanda | Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Sisitimu yePCR yeApplied Biosystems 7500 Real-Time QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yakagadzirwa neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.zvinokurudzirwa pakuburitsa sampuro uyematanho anotevera anofanira kunge arimafambiroyakaitwa zvichienderana nemutemo weIFUyeKit.
