Hepatitis B Virus DNA Quantitative Fluorescence
Zita rechigadzirwa
Kiti yekuongorora chirwere cheHepatitis B Virus DNA yeHWTS-HP015 (Fluorescence PCR)
Epidemiology
Hepatitis B chirwere chinokonzerwa nehutachiona hwehepatitis B (HBV), chinonyanya kuratidzwa nezvirwere zvinozvimba muchiropa, uye chinogona kukonzera kukuvara kwenhengo dzakawanda. Varwere veHepatitis B vanoratidzwa nekurapwa sekuneta, kushaya chido chekudya, kuzvimba kwemakumbo kana mativi ese, uye hepatomegaly nekuda kwekusashanda zvakanaka kwechiropa. Zvishanu muzana zvevanhu vakuru vane hutachiona uye 95% yevanhu vane hutachiona hwakamira havagone kubvisa HBV zvinobudirira, zvichikonzera hutachiona hunogara huripo, uye humwe hutachiona husingaperi hunozopedzisira hwava cirrhosis yechiropa uye hepatocellular carcinoma.[1-4].
Chiteshi
| FAM | HBV-DNA |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | serum itsva, Plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/mL |
| Kunyatsojeka | Mhedzisiro chaiyo inoratidza kuti zviitiko zvese makumi mashanu zvemasampuli eropa eHBV DNA asina hutano hazvina kunaka; mhinduro dzebvunzo dze cross-reactivity dzinoratidza kuti hapana kusangana pakati pekiti iyi nemamwe mavhairasi (HAV, HCV, DFV, HIV) pakutsvaga nucleic acid nemasampuli eropa, uye majini evanhu. |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Sisitimu yePCR yeLightCycler®480 Yenguva Yechokwadi Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemagetsi (Suzhou Molarray Co., Ltd.) Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Chinorayirwa chekuburitsa chinhu: Macro & Micro-Test Virus DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Kuburitsa chinhu kunofanirwa kuitwa zvichienderana nebhuku remirayiridzo, huwandu hwesampuro hwakabviswa i300μL, uye huwandu hweelution hunokurudzirwa i70μL.
