Hantaan Virus Nucleic
Zita rechigadzirwa
HWTS-FE005 Kiti yeKuona Hutachiona hweHantaan Virus Nucleic Acid (Fluorescence PCR)
Epidemiology
Hantavirus rudzi rwehutachiona hweRNA hwakaputirwa, hwakapatsanurwa, husina kusimba. Hantavirus yakakamurwa kuita mhando mbiri: imwe inokonzera Hantavirus pulmonary syndrome (HPS), uye imwe inokonzera Hantavirus hemorrhagic fever ine renal syndrome (HFRS). Yekutanga inonyanya kupararira muEurope neUnited States, uye yekupedzisira ihemorrhagic fever ine renal syndrome inokonzerwa neHantaan virus, iyo inowanzoonekwa muChina. Zviratidzo zvehantavirus hantaan type zvinonyanya kuoneka sehemorrhagic fever ine renal syndrome, iyo inoratidzwa nefivha yakanyanya, hypotension, kubuda ropa, oliguria kana polyuria uye kumwe kukanganisa kushanda kweitsvo. Inotapukira kuvanhu uye inofanira kutariswa zvakakwana.
Chiteshi
| FAM | hantavirus rudzi rwehantaan |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | seramu itsva |
| Ct | ≤38 |
| CV | ＜5.0% |
| LoD | Makopi 500/μL |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Zvinokurudzirwa kubvisa ma reagents: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubvisa kunofanirwa kuitwa zvichienderana neIFU. Vhoriyamu yekubvisa i200μL. Vhoriyamu yekudzikisa inokurudzirwa i80μL.
Zvinokurudzirwa kubvisa: Nucleic Acid Extraction kana Purification Kit (YDP315-R). Kubvisa kunofanirwa kuitwa zvichienderana neIFU. Vhoriyamu yekubvisa i140μL. Vhoriyamu yekubvisa inokurudzirwa i60μL.
