Chlamydia Trachomatis yakaomeswa nechando
Zita rechigadzirwa
HWTS-UR032C/D-Kit yeChlamydia Trachomatis Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification) yakaomeswa nechando
Epidemiology
Chlamydia trachomatis (CT) rudzi rwehutachiona hweprokaryotic hunowanikwa mumasero eukaryotic chete.[1]Chlamydia trachomatis yakakamurwa kuita maAK serotypes zvichienderana nenzira ye serotype. Utachiona hwemumaUrogenital tract hunowanzo kukonzerwa ne trachoma biological variant DK serotypes, uye varume vanowanzoonekwa se urethritis, iyo inogona kurapwa pasina kurapwa, asi yakawanda yacho inova isingaperi, inowedzerwa nguva nenguva, uye inogona kusanganiswa ne epididymitis, proctitis, nezvimwewo.[2]Vakadzi vanogona kukonzerwa neurethritis, cervicitis, nezvimwewo, uye mamwe matambudziko akakomba esalpingitis.[3].
Chiteshi
| FAM | Chlamydia trachomatis (CT) |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤30℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Chipfuko chemuromo wechibereko chemukadzi Chinobvisa urethra chemurume Weti yemurume |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Makopi mazana mana/mL |
| Kunyatsojeka | Hapana kusangana pakati pekit iyi nezvimwe zvirwere zve genitourinary tract infection zvakaita se high-risk Human papillomavirus type 16, Human papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei uye human genomic DNA, nezvimwewo. |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Munguva Yechokwadi (Hongshi Medical Technology Co., Ltd.) Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yekuona PCR yeLineGene 9600 Plus Real-Time (FQD-96A, tekinoroji yeHangzhou Bioer) MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Real-Time uye Sisitimu yePCR yeBioRad CFX Opus 96 Real-Time Sisitimu yekuona fluorescence iri nyore yeAmp chaiyo-nguva()HWTS-1600）. |
Kufamba Kwebasa
Sarudzo 1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kubviswa kunofanirwa kuitwa zvichienderana neIFU. Wedzera DNA yemuenzaniso yakatorwa nesample release reagent mu reaction buffer woedza pamudziyo zvakananga, kana kuti sampuro dzakatorwa dzinofanira kuchengetwa pa2-8℃ kweasingapfuuri maawa makumi maviri nemana.
Sarudzo yechipiri.
Kit yeDNA/RNA yeMacro & Micro-Test General (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kubviswa kunofanirwa kuitwa zvichienderana neIFU, uye huwandu hunokurudzirwa hwekubvisa i80μL. DNA yemuenzaniso inotorwa nenzira yemagnetic bead inopiswa pa95°C kwemaminitsi matatu uye ipapo inonyoroveswa nechando kwemaminitsi maviri. Wedzera DNA yemuenzaniso yakagadziriswa mu reaction buffer uye edza pamudziyo kana sampuli dzakagadziriswa dzinofanira kuchengetwa pasi pe -18°C kwemwedzi isingapfuuri mina. Huwandu hwekuomeswa nekunyungudika hunodzokororwa haufanirwe kudarika ma cycles mana.
