Virusi reFrost Encephalitis
Zita rechigadzirwa
HWTS-FE006 Forest Encephalitis Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Foresti encephalitis (FE), inozivikanwawo setick-borne encephalitis (tick-borne encephalitis, TBE), chirwere chinotapukira chetsinga dzemudumbu chinokonzerwa neforesti encephalitis virus. Foresti encephalitis virus iri muboka reFlavivirus remhuri yeFlaviviridae. Zvidimbu zvevhairasi iyi zvakaita sedenderedzwa rine dhayamita ye40-50nm. Huremu hwemamorekuru hunosvika 4×106Da, uye genome yehutachiona iRNA ine pfungwa yakanaka, ine tambo imwe chete[1]. Mukurapa, inoratidzwa nefivha yakanyanya, musoro, koma, kukurumidza kutanga kwekutsamwa kwemeningeal, uye kuoma mitezo yemutsipa nemakumbo, uye ine huwandu hwakawanda hwerufu. Kuongororwa nekukurumidza uye nekukurumidza kwehutachiona hweforest encephalitis ndiyo kiyi yekurapa forest encephalitis, uye kuiswa kwenzira iri nyore, chaiyo uye inokurumidza yekuongorora etiological inokosha zvikuru mukuongororwa kweforest encephalitis.[1,2].
Chiteshi
| FAM | hutachiona hwe encephalitis yemusango nucleic acid |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | seramu itsva |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/mL |
| Zvishandiso Zvinoshanda | Maitiro Ekushandisa Ezvinhu Zvehupenyu 7500 Masisitimu EPCR Enguva Yechokwadi Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Chinorayirwa kubvisa: Nucleic Acid Extraction kana Purification Kit (YDP315-R) neTiangen Biotech(Beijing) Co.,Ltd., kubvisa kunofanirwa kuitwa zvichienderana nemirairo. Vhoriyamu yemuenzaniso inotorwa i140μL uye vhoriyamu yekudzikisa i60μL.
Reagent inokurudzirwa yekuburitsa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). Kuburitsa kunofanirwa kuitwa zvichienderana nemirairo. Vhoriyamu yemuenzaniso inotorwa i200μL uye vhoriyamu yekudzikisa inokurudzirwa i80μL.
