Enterovirus Universal, EV71 uye CoxA16
Product Name
HWTS-EV026B-Enterovirus Universal, EV71 uye CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
HWTS-EV020Y/Z-Freeze-yakaomeswa Enterovirus Universal, EV71 uye CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
Chitupa
CE/MDA (HWTS-EV026)
Epidemiology
Hand-foot-mouth disease (HFMD) chirwere chinotapukira chinotapukira muvana. Inonyanya kuitika muvana vari pasi pemakore mashanu, uye inogona kukonzera herpes pamaoko, tsoka, muromo uye zvimwe zvikamu, uye nhamba shomanana yevana inogona kukonzera matambudziko akadai semyocarditis, pulmonary edema, aseptic meningoencephalitis, etc. Vana vari voga vane zvirwere zvakakomba vanoderera nokukurumidza uye vanofa kana vasina kurapwa nokukurumidza.
Parizvino, 108 serotypes ye enteroviruses yakawanikwa, iyo yakakamurwa kuva mapoka mana: A, B, C uye D. Enteroviruses inokonzera HFMD yakasiyana-siyana, asi enterovirus 71 (EV71) uye coxsackievirus A16 (CoxA16) ndiyo inowanzozivikanwa uye kuwedzera kune HFMD, inogona kukonzera zvakakomba zvakadai se-central nervous system, complication, encephalitis, uye complication.
Channel
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Kudzora mukati |
Technical Parameters
| Storage | Mvura: ≤-18℃ MurimaLyophilization: ≤30 ℃ |
| Sherufu-hupenyu | Mvura: 9 mwedziLyophilization: 12 mwedzi |
| Type Type | Sample yehuro, Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500Makopi/mL |
| Zvishandiso Zvishandiso | Inogona kuenderana neyakajairwa fluorescent PCR zviridzwa pamusika.ABI 7500 Chaiyo-Nguva PCR Systems ABI 7500 Inokurumidza Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems QuantStudio®5 Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR Systems LineGene 9600 Plus Chaiyo-Nguva PCR Detection Systems MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Total PCR Solution
Inokurudzirwa kuburitsa reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Iyo yakabviswa sampuli vhoriyamu i200μL, uye yakakurudzirwa elution vhoriyamu i80μL.
Inokurudzirwa kuburitsa reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kubvisa kunofanira kuitwa maererano nebhuku rekuraira. Iwo masampula ekuwedzera ndeye oropharyngeal swabs kana herpes fluid samples yevarwere vakaunganidza panzvimbo. Wedzera iyo yakaunganidzwa swabs yakananga kuMacro & Micro-Test Sample Release Reagent, vortex uye sanganisa zvakanaka, isa panzvimbo yekupisa kwemaminetsi mashanu, tora kunze uye wozopinza uye sanganisa zvakanaka kuti uwane iyo RNA yemuenzaniso wega wega.
Inokurudzirwa kubvisa reagent: QIAamp Viral RNA Mini Kit (52904) neQIAGEN kana Nucleic Acid Extraction kana Purification Reagent (YDP315-R). Kubvisa kunofanira kuitwa zvakanyatsoenderana nebhuku rekuraira.
