Enterovirus Universal, EV71 uye CoxA16
Zita rechigadzirwa
HWTS-EV026B-Enterovirus Universal, EV71 uye CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-EV020Y/Z-Kit yekuongorora Enterovirus Universal, EV71 neCoxA16 Nucleic Acid (Fluorescence PCR) yakaomeswa nechando
Chitupa
CE/MDA（HWTS-EV026）
Epidemiology
Chirwere chemuromo wemaoko (HFMD) chirwere chinotapukira chinowanzoitika kuvana. Chinowanzoonekwa kuvana vari pasi pemakore mashanu, uye chinogona kukonzera herpes mumaoko, tsoka, muromo nedzimwe nhengo, uye vana vashoma vanogona kukonzera matambudziko akadai se myocarditis, pulmonary edema, aseptic meningoencephalitis, nezvimwewo. Vana vane zvirwere zvakakomba vanorwara nekukurumidza uye vanogona kufa kana vakasarapwa nekukurumidza.
Parizvino, ma serotypes zana nemasere ema enterovirus akawanikwa, ayo akakamurwa kuita mapoka mana: A, B, C naD. Ma Enterovirus anokonzera HFMD akasiyana, asi enterovirus 71 (EV71) ne coxsackievirus A16 (CoxA16) ndiwo anonyanya kuwanikwa uye pamusoro peHFMD, anogona kukonzera matambudziko makuru etsinga dzemutsinga dzakadai se meningitis, encephalitis, uye acute flaccid paralysis.
Chiteshi
| FAM | Hutachiona hweEnterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Kudzora kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | Mvura: ≤-18℃ murimaKupisa kweropa: ≤30℃ |
| Nguva yekuzorora | Mvura: mwedzi 9Kugadziriswa kweropa: mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | Muenzaniso wehuro, Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Makopi 500/mL |
| Zvishandiso Zvinoshanda | Inogona kuenzana nemidziyo yePCR inopenya iri pamusika.Masisitimu eABI 7500 PCR Enguva Yechokwadi Masisitimu ePCR eABI 7500 Anokurumidza Kushanda Munguva Yechokwadi Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Masisitimu ePCR eQuantStudio®5 Enguva Yechokwadi Masisitimu ePCR eLightCycler®480 Munguva Yechokwadi Masisitimu ekuona maPCR eLineGene 9600 Plus Real-Time MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Mhinduro yePCR Yese
Chinorayirwa kubvisa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubvisa kunofanirwa kuitwa zvichienderana nebhuku remirayiridzo. Vhoriyamu yemuenzaniso yakabviswa i200μL, uye vhoriyamu yekudzikisa inokurudzirwa i80μL.
Chinorayirwa kubvisa mushonga: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kubvisa mushonga kunofanira kuitwa zvichienderana negwaro remirayiridzo. Mienzaniso yekuburitsa mushonga ndeye oropharyngeal swabs kana herpes fluid samples dzevarwere vakaunganidzwa panzvimbo yacho. Wedzera mushonga wakaunganidzwa zvakananga kuMacro & Micro-Test Sample Release Reagent, vortex wosanganisa zvakanaka, isa patembiricha yemumba kwemaminitsi mashanu, bvisa wobva wachinja wosanganisa zvakanaka kuti uwane RNA yemuenzaniso wega wega.
Chinorayirwa kubvisa: QIAamp Viral RNA Mini Kit (52904) neQIAGEN kana Nucleic Acid Extraction kana Purification Reagent (YDP315-R). Kubvisa kunofanirwa kuitwa zvichienderana negwaro remirayiridzo.
