Virusi reDengue, Virusi reZika uye Chikungunya Virus Multiplex
Zita rechigadzirwa
HWTS-FE040 Dengue Virus, Zika Virus uye Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Dengue fever (DF), iyo inokonzerwa nehutachiona hwedengue virus (DENV), ndeimwe yezvirwere zvinotapukira zve arbovirus zvakanyanya kupararira. Nzira yayo yekutapurirana inosanganisira Aedes aegypti neAedes albopictus. DF inonyanya kuwanikwa munzvimbo dzinopisa uye dziri pasi pemvura. DENV inhengo ye flavivirus iri pasi pe flaviviridae, uye inogona kuiswa muzvikamu zvina zvichienderana ne surface antigen. Zviratidzo zvechirwere cheDENV zvinosanganisira musoro, fivha, kushaya simba, kuwedzera kwe lymph node, leukopenia nezvimwewo, uye kubuda ropa, shock, kukuvara kwechiropa kana kunyange kufa muzviitiko zvakakomba. Mumakore achangopfuura, shanduko yemamiriro ekunze, kukura kwemaguta, kukura nekukurumidza kwevashanyi nezvimwe zvinhu zvakapa mamiriro ekukurumidza uye ari nyore ekutapurirana nekupararira kweDF, zvichikonzera kuwedzera nguva dzose kwenzvimbo yedenda yeDF.
Chiteshi
| FAM | DENV nucleic acid |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | -18℃ |
| Nguva yekuzorora | mwedzi mipfumbamwe |
| Rudzi rwemuenzaniso | Seramu itsva |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | Makopi 500/mL |
| Kunyatsojeka | Mhedzisiro yebvunzo yekupindira inoratidza kuti kana huwandu hwebilirubin muropa husingapfuure 168.2μmol/ml, huwandu hwehemoglobin hunogadzirwa nehemolysis husingapfuure 130g/L, huwandu hwemafuta muropa husingapfuure 65mmol/ml, huwandu hwese hweIgG muropa husingapfuure 5mg/mL, hapana mhedzisiro pahutachiona hwedengue, kuonekwa kwehutachiona hweZika kana chikungunya. Hutachiona hweHepatitis A, hutachiona hweHepatitis B, hutachiona hweHepatitis C, hutachiona hweHerpes, hutachiona hweEastern equine encephalitis, Hantavirus, hutachiona hweBunya, hutachiona hweWest Nile uye samples dze serum dzemajini evanhu dzinosarudzwa kuti dziongororwe cross-reactivity, uye mhedzisiro yacho inoratidza kuti hapana cross reaction pakati pekit iyi nehutachiona hwataurwa pamusoro apa. |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Sarudzo 1.
TIANamp Virus DNA/RNA Kit (YDP315-R), uye kubviswa kwayo kunofanira kuitwa zvichienderana nemirairo yekushandisa. Vhoriyamu yemuenzaniso yakatorwa i140μL, uye vhoriyamu yekubvisa inokurudzirwa i60μL.
Sarudzo yechipiri.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd., uye kubviswa kwayo kunofanira kuitwa zvichienderana nemirairo yekushandisa. Vhoriyamu yemuenzaniso yakabviswa i200μL, uye vhoriyamu yekudzikisa inokurudzirwa i80μL.
