Dengue Virus, Zika Virus uye Chikungunya Virus Multiplex
Product name
HWTS-FE040 Dengue Virus, Zika Virus uye Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Dengue fever (DF), iyo inokonzerwa nehutachiona hwedengue (DENV) hutachiona, ndeimwe yezvirwere zvakanyanya kupararira arbovirus. Iyo yekufambisa svikiro inosanganisira Aedes aegypti uye Aedes albopictus. DF inonyanya kuwanda munzvimbo dzinopisa uye dzinopisa. DENV ndeye flavivirus pasi pe flaviviridae, uye inogona kuiswa mu 4 serotypes zvichienderana nepamusoro antigen. Kuratidzira kwekiriniki yehutachiona hweDENV kunonyanya kusanganisira kutemwa nemusoro, fivha, kushaya simba, kuwedzera lymph node, leukopenia uye nezvimwe, uye kubuda ropa, kuvhunduka, kukuvara kwechiropa kana kunyange kufa mumatambudziko akaomarara. Mumakore achangopfuura, shanduko yemamiriro ekunze, kushanya kwemadhorobha, kukurumidza kusimudzira kushanya uye zvimwe zvinhu zvakapa mamiriro ekukurumidza uye ari nyore ekufambisa nekuparadzira DF, zvichikonzera kuwedzera kwedenda nzvimbo yeDF.
Channel
| FAM | DENV nucleic acid |
| ROX | Kudzora Kwemukati |
Technical Parameters
| Storage | -18 ℃ |
| Sherufu-hupenyu | 9 mwedzi |
| Type Type | Fresh serum |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 Makopi/mL |
| Zvakananga | Kupindira kwekuongororwa kwemigumisiro inoratidza kuti kana huwandu hwebilirubin mu serum isingapfuuri 168.2μmol / ml, hemoglobin concentration inobudiswa nehemolysis haisi kupfuura 130g / L, ropa re lipid concentration haisi kupfuura 65mmol / ml, iyo yose yeIgG muserum haisi kupfuura 5mg / mL, hapana hutachiona hweZika kana chikungue hutachiona. Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus uye human genomic serum samples inosarudzwa pamuchinjikwa-reactivity bvunzo, uye mhedzisiro inoratidza kuti hapana kupindirana pakati pekiti iyi uye hutachiona hwataurwa pamusoro apa. |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
TIANamp Virus DNA/RNA Kit (YDP315-R), uye kubviswa kunofanirwa kuitwa zvichinyatsoenderana nekuraira kwekushandisa. Iyo yakabviswa sampuli vhoriyamu i140μL, uye yakakurudzirwa elution vhoriyamu i60μL.
Sarudzo 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (iyo inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HCTS0, HWTS-30B) & Micro-Test Med-Tech Co., Ltd., uye kudhirowa kunofanirwa kuitwa zvinoenderana nekuraira kwekushandisa. Iyo yakabviswa sampuli vhoriyamu i200μL, uye yakakurudzirwa elution vhoriyamu i80μL.
