Dengue virus, zika virus and chirunguya virus kuwanda
Chigadzirwa Zita
HWTS-FE040 Denga Virus, Zika Virus And Chikugunya Virus Multidic Accation Actic Kuona Kit (Fluorescence PCR)
Epidemiology
DENEUE Fever (DF), iyo inokurudzirwa neDhenue virus (Denv) utachiona, ndeimwe yedenda renzvimbo hosha dzinotapukira. Yayo yekufambisa yepakati inosanganisira AEDES AELAGSI uye AEDES ALBOPIFTUS. DF inonyanya kuve yakapararira munzvimbo dzinopisa uye dzishoma. Denv ndeyaplavivirus pasi peFlavivuridae, uye inogona kuiswa muzvikamu zvina zvichienderana nekumusoro antigen. Kuratidzwa kwekiriniki kweDenv utachiona kunonyanya kusanganisira musoro, fivha, kushaya simba kweLymph node, leukopenia uye etching, kuvakuvadza, kushungurudzika, kunyange kufa munzvimbo dzakakura. Mumakore achangopfuura, shanduko yemamiriro ekunze, kukura kwemamiriro ekunze kwekushanya uye zvimwe zvinhu zvakakurudzira nekukurumidza uye zvakaringana zvekuparadzira uye zvichitungamira
Chiteshi
| Nhamo | Denv nucleic acid |
| Rox | Kutonga kwemukati |
Technical parameter
| Chengetedza | -18 ℃ |
| Shefu-Hupenyu | 9 mwedzi |
| Type Type | Serum nyowani |
| Ct | ≤38 |
| CV | <5% |
| Lod | 500 makopi / ml |
| Chokwadi | Kuvhiringidza Kwekupindira Mhedzisiro Inoratidza kuti kana iko kusungwa kweBilirubin muSerum hakusati kwaitwa neLemolysis hakusi kweJillisration kusunganidzwa muSerum hakusi kupfuura 5mg / ml, hapana chakaipa pane hutachiona hwehutachiona, zika hutachiona kana Chikungunya virus kuona. Hepatitis hutachiona, Hepatitis Chutsi, Heppise Curus, Heppes Virus, Equinens Equinenlitis Virus, Hantavirus, Bun Hilus Sampuli Sampuli uye Elomic Sampoliss Cross maitiro pakati peiyi kit uye vafambi vakataurwa pamusoro apa. |
| Zviridzwa zvinoshanda | Yakaiswa Biosystems 7500 chaiyo-nguva PCR system Yakaiswa biosystems 7500 nekukurumidza chaiyo-nguva pcr system Quantstudio®5 chaiyo-nguva PCR system Mwenje wechiedza®480 chaiyo-nguva PCR system Linegene 9600 Plus Real-Nguva PRR Kuongororwa system Ma-6000 chaiyo-nguva huwandu hweThebrary cyler Biorad cfx96 chaiyo-nguva pcr system Biorad cfx opus 96 chaiyo-nguva pcr system |
Shanda kuyerera
Sarudzo 1.
Tianamp turus DNA / rna kit (ydp315-r), uye kubviswa kunofanirwa kuitwa mukunyatsoenderana nemirairo yekushandiswa. Iyo inobviswa sampuli vhoriyamu ndeye 140μL, uye iyo Yakakurudzirwa Vhoriyamu Vhoriyamu ndeye60μL.
Sarudzo 2.
Macro & Micro-Test General DNA / rna Kit (HWTS-3017-50, HWTS-3017-48, HWTS-3017-96) (Micro-test Autocatic Acclec Excractor (HWTS-3006C, HWTS-3006B)) NaJiangsu Macro & Micro-Test Med-Tech Co, Ltd., uye The the kubviswa kunofanirwa kuitiswa maererano nemirairo yekushandiswa. Iyo yakabviswa sampuli vhoriyamu ndeye 200μl, uye iyo yakakurudzirwa elution vhoriyamu ndeye 80μl.
