Coxsackie Virus Type A16 Nucleic Acid

Iyi kit inotora Enzymatic Probe Isothermal Amplification (EPIA), uye inogadzira chaiyo primers uye RNA base-ine probes (rProbe) yenzvimbo yakachengetedzwa zvakanyanya yeCox A16, uye inowedzera Bst enzyme uye RNaseH enzyme panguva imwe chete, umo kuruboshwe uye. migumo yekurudyi yeRNA base yerProbe yakanyorwa nemapoka efluorescent uye quencher, zvichiteerana.Shandisa iyo DNA polymerase chiitiko uye strand displacement chiitiko cheBst enzyme kukwidziridza chinangwa kuti chiongororwe pakupisa nguva dzose, iyo RNaseH enzyme inogona kutsemura mabhesi eRNA pane inotangwa-probe hybrid cheni, kuti boka refluorescent uye quencher yerProbe ive. akaparadzaniswa nokudaro fluorescing.Pamusoro pezvo, chimedu chekuruboshwe cheyakasara rProbe RNA base inogona kushandiswa seprimer kuti iwedzere kuwedzera kugadzira chigadzirwa, icho chinowedzera kuunganidza chigadzirwa.Chiratidzo chefluorescent chinoramba chichiunganidzwa nekugadzirwa kwechigadzirwa, nokudaro nekuona kuonekwa kwechinangwa che nucleic acid.