Chikungunya Virus IgM/IgG Antibody Detection Kit (Colloidal gold)
Zita rechigadzirwa
Chikungunya Virus IgM/IgG Antibody Detection Kit (Colloidal gold)
Epidemiology
Chikungunya chirwere chinotapuriranwa zvakanyanya chinokonzerwa nehutachiona hwechikungunya (CHIKV), chinotapuriranwa neumhutu hweAedes, uye chinoratidzwa nefivha, mapundu uye kurwadziwa kwemajoini. Chikungunya yakasimbiswa kuti yakajairika muTanzania muna 1952, uye hutachiona hwakawanikwa muna 1956. Chirwere ichi chakanyanya kuparariramuAfrica nekuSoutheast Asia, uye zvakakonzera denda guru munharaunda yeIndian Ocean mumakore achangopfuura [1]. Utachiona hwechikungunya hune mhando imwe uye hunogona kukamurwa kuita majini matatu, anoti rudzi rweWest Africa, rudzi rweCentral-East-Southern Africa uye rudzi rweAsia [2]. Utachiona hwechikungunya hwave kuwanikwa munyika dzinopfuura 110 muAsia, Africa, Europe neAmerica [3]. Zviratidzo zvechirwere zvakafanana nedengue fever, uye zviri nyore kuongororwa zvisizvo. Kunyangwe huwandu hwerufu hwakaderera, zviri nyore kugadzira kubuda kukuru uye denda munzvimbo dzine humhutu hwakawanda [4]. IgM uye IgG antibodies muropa rose revanhu, serum, kana plasma samples, uye inogona kushandiswa sechishandiso chekubatsira pakuongorora hutachiona hwechikungunya pakutanga mukiriniki. Kit iyi yakakodzera kuona maantibodies eChikungunya muvanhu vane zviratidzo zvakasiyana.
Magadzirirwo ehunyanzvi
| Kuchengetera | 4~30℃ |
| Nguva yekuzorora | mwedzi makumi maviri nemana |
| Rudzi rwemuenzaniso | Serum, plasma, uye sampuli yeropa rose |
| LoD | Kuona IgM: kunzwisisika 97.56%, kujeka98.74%, huwandu hwese hwezviitiko zvakangoitika 98.50%. Kuona IgG: kunzwa 97.67%, specificity 98.73%, total coincidence rate 98.50%. |
| Kunyatsojeka | Hapana cross-reactivity yakaonekwa muongororo dzecross-reactivity dzekit iyi ineVirusi reJapan re encephalitis, hutachiona hwe encephalitis hunotapukira nezvikwekwe, fivha yakakura ine hutachiona hwe thrombocytopenia syndrome, hutachiona hweXinjiang hemorrhagic fever, Hantavirus, Dengue virus, Hepatitis C virus, Influenza A virus uye Influenza Hutachiona hweB. |
Kufamba kwebasa:
Ndapota verengai bhuku remirayiridzo nokungwarira musati maedza. Musati maedza, bvisai ma reagents ese nemasamples kuti ayedzwe kubva mumamiriro ekuchengetera moarega adzoke patembiricha yemukamuri. Bvunzo iyi inofanira kuitwa patembiricha yemukamuri.
Serum, plasma, uye sampuli yeropa rose:
1. Bvisa kadhi rekuyedza mubhegi realuminium foil woriisa panzvimbo yakati sandara uye yakaoma;
2. Shandisa pipette kuwedzera donhwe rimwe chete re serum, plasma, kana ropa rose (rinenge 10µL) mutsime remuenzaniso "S";
3. Wobva wawedzera madonhwe maviri emusanganiswa wemuenzaniso (anenge 70 µL) mutsime remuenzaniso "S";
4. Sarudza mhedzisiro mukati memaminitsi gumi nemashanu kusvika makumi maviri. Mhedzisiro inoratidzwa mushure memaminitsi makumi maviri haishande.
