ALDH Kuumbwa kwemajini
Zita rechigadzirwa
HWTS-GE015Kiti yeKuona Kuwanikwa kweMajini eAlDH (ARMS -PCR)
Epidemiology
Jini reALDH2 (acetaldehyde dehydrogenase 2), rinowanikwa pachromosome yemunhu 12. ALDH2 ine esterase, dehydrogenase uye reductase activity panguva imwe chete. Zvidzidzo zvakaratidza kuti ALDH2 ienzyme ye metabolic ye nitroglycerin, iyo inoshandura nitroglycerin kuita nitric oxide, nokudaro ichizorodza mitsipa yeropa uye ichivandudza matambudziko ekuyerera kweropa. Zvisinei, kune polymorphisms mujini reALDH2, iro rinonyanya kuwanika kuEast Asia. Wild-type ALDH2*1/*1 GG ine hunyanzvi hwakasimba hwe metabolic, nepo heterozygous type ine 6% chete ye wild-type enzyme activity, uye homozygous mutant type haina enzyme activity, ine metabolism isina simba zvakanyanya uye haigone kuita mhedzisiro yaidiwa, nokudaro ichikonzera kukuvadza muviri wemunhu.
Chiteshi
| FAM | ALDH2 |
| ROX | Kudzora Kwemukati |
Magadzirirwo ehunyanzvi
| Kuchengetera | ≤-18℃ |
| Nguva yekuzorora | mwedzi gumi nemiviri |
| Rudzi rwemuenzaniso | EDTA ropa risingaunganidzi ropa |
| CV | <5.0% |
| LoD | 103Makopi/mL |
| Zvishandiso Zvinoshanda | Sisitimu yePCR yeApplied Biosystems 7500 Real-Time Maitiro eApplied Biosystems 7500 PCR Systems Inokurumidza Munguva Yechokwadi QuantStudio®Masisitimu mashanu ePCR enguva chaiyo Masisitimu ePCR eSLAN-96P Enguva Yechokwadi Chiedza cheKufambisa Mwenje®Sisitimu yePCR ye480 Real-Time Sisitimu yeKuona PCR yeLineGene 9600 Plus Yenguva Yechokwadi MA-6000 Chaiyo-Nguva Yekuyera Kupisa Kwemhepo Sisitimu yePCR yeBioRad CFX96 Yenguva Yechokwadi Sisitimu yePCR yeBioRad CFX Opus 96 Yenguva Yechokwadi |
Kufamba Kwebasa
Zvinokurudzirwa kuti ubvise mareagents: shandisa Blood Genome DNA Extraction Kit (DP318) yakagadzirwa neTiangen Biotech (Beijing) Co., Ltd. kana Blood Genome Extraction Kit (A1120) yakagadzirwa nePromega kuti ubvise EDTA yeropa risingaunganidzi ropa.
Zvinokurudzirwa kubvisa mareagents: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubvisa kunofanirwa kuitwa zvichienderana nemirayiridzo. Huwandu hwekudzikisa hunokurudzirwa ndi100μL.
