Mhando makumi maviri nemasere dzeHuman Papillomavirus (16/18/45Typing) Nucleic Acid Detection Kit (Fluorescence PCR)

Tsananguro pfupi:

[ChigadzirwaKodhi] HWTS-CC024

[Kushandiswa Kwakarongwa]

Kit iyi yakakodzera kuonekwa kwemhando makumi maviri nemasere dzehutachiona hwepapillomavirus (HPV 6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) nucleic acidsmukirinikiwetimuenzaniso,muenzaniso weswab yemuromo wemukadzi uye muenzaniso weswab yemukadzi yekuberekapamwe neHPV 16/18/45 kuonekwa kwekunyora, panguva iyomamwe marudzi haagoni kuva zvachoseakasiyana, kuparubatsiro mukuongororwa uye kurapwa kwehutachiona hweHPV.

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Mhando makumi maviri nemasere dzeHuman Papillomavirus (16/18/45Typing) Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Human Papillomavirus (HPV) ndeyemhuri yePapillomaviridae yehutachiona hweDNA hudiki, husina kuvharwa, hwakatenderera kaviri, hune genome hunosvika 8000 base pairs (bp). HPV inotapukira vanhu kuburikidza nekubata zvakananga kana zvisina kunanga zvinhu zvakasvibiswa kana kutapurirana pabonde. Hutachiona uhwu hausi hwemunhu chete, asiwo hune nyama, uye hunogona kutapurira ganda revanhu nemasero emucosal epithelial, zvichikonzera mhando dzakasiyana dzemapillomas kana maronda paganda revanhu uye kukuvara kuri kuwedzera kwe epithelium yekubereka.

Gomarara remumuromo wechibereko ndeimwe yemabundu anonyanya kuipa munhengo dzekubereka dzevakadzi. Zvidzidzo zvakaratidza kuti hutachiona hweHPV hunoramba huripo uye hutachiona hwakawanda ndizvo zvimwe zvezvikonzero zvikuru zvegomarara remumuromo wechibereko. Parizvino, mishonga inoshanda inozivikanwa ichiri kushaikwa yegomarara remumuromo wechibereko rinokonzerwa neHPV, saka kuwanikwa nekukurumidza uye kudzivirira hutachiona hwemumuromo wechibereko hunokonzerwa neHPV ndiyo kiyi yekudzivirira gomarara remumuromo wechibereko. Zvakakosha zvikuru kugadzira bvunzo iri nyore, chaiyo uye inokurumidza yekuongorora chirwere chegomarara remumuromo wechibereko kuti rionekwe uye rirapwe.

Magadzirirwo ehunyanzvi

Kuchengetera	≤ -18℃
Nguva yekuzorora	mwedzi gumi nemiviri
Rudzi rwemuenzaniso	Uwainimuenzaniso,Fsampuro ye emale cervical swab, Fsampuro ye swab yekubereka yemukadzi yeEmale
CV	<5.0%
LoD	Muganho weKuona (LoD): LoD yekiti iyi i300Cmaopies/mL
Kunyatsojeka	Hapana kusangana kwemarudzi akasiyananezvirwere zvakafanana.
Zvishandiso Zvinoshanda	Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A,HangzhouTekinoroji yeBioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System uye BioRad CFX Opus 96 Real-Time PCR System.

Chiteshi:

Reaction Buffer	Fluorophore	Mudzimisi
PCR-Mix1/PCR-Mix2	FAM	hapana
	VIC(HEX)	hapana
	ROX	hapana
	CY5	hapana

Kufamba kwebasa: Ona Kit IFU kuti uwane rumwe ruzivo

Mhinduro 1 (Kubviswa kwe nucleic acid otomatiki)

Mhinduro 2: (Macro & Micro-Test Sample Release Reagent)

Ma reagents anoda asi haana kupihwa:Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) neJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).

Zvinhu zvinoshandiswa zvinodiwa asi hazvipihwe: 1.5mL DNase/RNase isinacchinhu chinoparadzatmabhesi,DMhuno/RZvishandiso zvisina Nase, 0.2mL yemachubhu ePCR, centrifuge yedesktop, musanganiswa weoscillation yedesktop.